Figure 5: Determination of F3- and LE-induced alteration of protein-level expression of apoptosis-related factors .Total protein was extracted and pro-caspase 3, pro-caspase 9, Bak, PARP, cleaved- PARP, XIAP, Nuclear AIF, Mitochondrial AIF, Bcl-2, Bax were analyzed by Western blot. GAPDH was used as an internal control. PARP = poly (ADP-ribose) polymerase; XIAP = X-lined inhibition of apoptosis; AIF = apoptosis-inducing factor. Each experiment was performed in triplicate.*p < 0.05 vs. control (0 μM).