Lipase Human Mouse Frog Human Mouse Frog Human Mouse Frog Human Human Mouse Human Mouse Human Mouse Seasquirt PTL PTL PTL PLR1 PLR1 PLR1 PLR2 PLR2 PLR2 PLR3 HL HL EL EL LPL LPL Lipase Human PTL 100 78 55 67 67 64 64 68 49 47 25 26 27 27 24 24 35 Mouse PTL 78 100 54 67 66 62 62 69 47 45 25 27 26 27 22 24 34 Frog PTL 55 54 100 52 54 60 52 53 53 46 24 27 26 27 24 24 35 Human PLR1 67 67 52 100 83 60 62 63 47 48 29 30 29 26 26 25 36 Mouse PLR1 67 66 54 83 100 60 62 63 49 49 29 29 27 27 26 26 36 Frog PLR1 64 62 60 60 60 100 58 61 56 47 24 25 28 26 23 23 37 Human PLR2 64 62 52 62 62 58 100 74 47 47 27 26 25 25 24 24 36 Mouse PLR2 68 69 53 63 63 61 74 100 43 47 29 27 25 27 24 22 32 Frog PLR2 49 47 53 47 49 56 47 43 100 43 25 26 23 25 22 22 32 Human PLR3 47 45 46 48 49 47 47 47 43 100 29 27 22 27 24 22 32 Human HL 25 25 24 29 29 24 27 29 25 29 100 74 38 42 44 42 21 Mouse HL 26 27 27 30 29 25 26 27 26 27 74 100 42 40 44 43 25 Human EL 27 26 26 29 27 28 25 25 23 25 38 42 100 80 44 45 25 Mouse EL 27 27 27 26 27 26 25 27 23 27 42 40 80 100 45 46 25 Human LPL 24 22 24 26 26 23 24 24 22 24 44 44 44 45 100 92 25 Mouse LPL 24 24 24 25 26 23 24 22 23 22 42 43 45 46 92 100 25 Seasquirt Lipase 35 34 35 36 36 37 36 32 32 32 21 25 25 25 25 25 100

Table 2: Percentage Identities for Vertebrate and Sea Squirt Lipase Amino Acid Sequences. Numbers show the percentage of amino acid sequence identities. Numbers in bold show higher sequence identities for lipases from the same gene family: PTL; PLR1; PLR2; PLR3; HL, EL and LPL represent genes encoding hepatic lipase, endothelial lipase and lipoprotein lipase, respectively.