| Position in gel |
Protein Namea |
Genename / Site number |
Access Number |
pI / M.W |
Metabolismb pathway |
Signal
Peptidec |
Subcellulard
localization |
| Point 1 |
maltose binding protein |
BMEI1716 |
NP_540633.1 |
5.09/46228 |
transport |
no |
P |
| Point 3 |
isovalery-CoAdehydrogenase |
ivd |
NP_540840.1 |
5.36/41428 |
metabolic process |
no |
C |
| Point 5 |
25kDa-outer membrane immunogenic protein precursor |
BMEI1829 |
NP_540746.1 |
4.78/24571 |
transport |
yes |
OM |
| Point 6 |
31kDa-outer membrane
immunogenic protein precursor |
omp31 |
NP_541822.1 |
5.21/23258 |
Ion transport |
no |
OM |
| Point 7 |
glycerol trinitrate reductase |
BMEII0394 |
NP_541372.1 |
5.80/39981 |
metabolic process |
no |
C |
| Point 8 |
succinyl-CoA synthetase
subunit alpha |
BMEI0139 |
NP_539057.1 |
5.81/31214 |
tricarboxylic acid cycle |
no |
C |
| Point 10 |
peptidyl-prolyl cis-trans
isomerase A |
PPI |
NP_539805.1 |
6.08/19145 |
protein processing |
no |
OM |
| Point 9 |
S-adenosyl-L-
homocysteine hydrolase |
ahcY |
NP_540946.1 |
5.30/52430 |
Amino-acid biosynthesis |
no |
C |
| Point 2 |
Protein translation elongation
factor EF-TU |
tufA/tufB |
AAL51923.1 |
5.60/44313 |
protein biosynthesis |
no |
C |
The immunodominant protein dots were subjected to trypase digestion and LC-MS/MS identification. The results were subjected to bioinformation retrieval, signal peptide
prediction, subcellular localizations confirmation and searching for metabolism pathways.
a. The Arabic numbers are concord with the gel and PVDF membrane.
b. The metabolism pathways of identified proteins were searched in the Web of http://www.kegg.com/.
c. Signal peptide prediction was accomplished through SignalP 3.0 software (http://www.cbs.dtu.dk/services/SignalP/). “+”,with signaling peptide; “-” without signaling peptide.
d. Subcellular localizations were predicted by PSORTb v2.0 software (http://www.psort.org/). C, Cytoplasmic; P, Periplasmic; OM, Outer Membrane |
