Position in gel Protein Namea Genename / Site number Access Number pI / M.W Metabolismb pathway Signal
Point 1 maltose binding protein BMEI1716 NP_540633.1 5.09/46228 transport no P
Point 3 isovalery-CoAdehydrogenase ivd NP_540840.1 5.36/41428 metabolic process no C
Point 5 25kDa-outer membrane immunogenic protein precursor BMEI1829 NP_540746.1 4.78/24571 transport yes OM
Point 6 31kDa-outer membrane
immunogenic protein precursor
omp31 NP_541822.1 5.21/23258 Ion transport no OM
Point 7 glycerol trinitrate reductase BMEII0394 NP_541372.1 5.80/39981 metabolic process no C
Point 8 succinyl-CoA synthetase
subunit alpha
BMEI0139 NP_539057.1 5.81/31214 tricarboxylic acid cycle no C
Point 10 peptidyl-prolyl cis-trans
isomerase A
PPI NP_539805.1 6.08/19145 protein processing no OM
Point 9 S-adenosyl-L-
homocysteine hydrolase
ahcY NP_540946.1 5.30/52430 Amino-acid biosynthesis no C
Point 2 Protein translation elongation
factor EF-TU
tufA/tufB AAL51923.1 5.60/44313 protein biosynthesis no C
The immunodominant protein dots were subjected to trypase digestion and LC-MS/MS identification. The results were subjected to bioinformation retrieval, signal peptide prediction, subcellular localizations confirmation and searching for metabolism pathways.
a. The Arabic numbers are concord with the gel and PVDF membrane.
b. The metabolism pathways of identified proteins were searched in the Web of http://www.kegg.com/.
c. Signal peptide prediction was accomplished through SignalP 3.0 software (http://www.cbs.dtu.dk/services/SignalP/). “+”,with signaling peptide; “-” without signaling peptide.
d. Subcellular localizations were predicted by PSORTb v2.0 software (http://www.psort.org/). C, Cytoplasmic; P, Periplasmic; OM, Outer Membrane
Table 1: The results for LC-MS/MS identification and information retrieval on immunoreactive proteins.