Primers number |
Primer sequences(5’-3’) |
protein names and position in gel |
Length of PCR
products (bp) |
PCR conditions (°C/s) |
PCR results of four strains of Brucella |
Denaturation |
Annealing |
Elongation |
M5 |
16M |
544A |
S19 |
1 |
catatgatgggaattgccccggcttac
ctcgagtcaaaggtcgccggcgtcttc |
maltose binding protein (point 1) |
1272 |
94/30 |
60/30 |
72/60 |
+ |
+ |
+ |
+ |
2 |
catatgatgaacttcggtcttggcgag
ctcgagtcaacgggtctcctgaaagag |
isovalery-CoA dehydrogenase
(point 3) |
1149 |
94/30 |
58/30 |
72/60 |
+ |
+ |
+ |
+ |
3 |
catatgatgaagctcaaggctcttc
ctcgagtcagaacttgtaagcgacac |
25kDa-outer membrane immunogenic protein precursor (point 5) |
687 |
94/30 |
55/30 |
72/60 |
+ |
+ |
+ |
+ |
4 |
catatggtggttgtttctgaaccttccg
ctcgagttagaacttgtagttcagaccgacg |
31kDa-outer membrane
immunogenic protein precursor
(point 6) |
660 |
94/30 |
58/30 |
72/60 |
+ |
+ |
- |
- |
5 |
gagctcatggcctcattgttcgaccccattac
ctcgag tcatgccagaacgggataatc |
glycerol trinitrate reductase (point7) |
1116 |
94/30 |
58/30 |
72/60 |
+ |
+ |
+ |
+ |
6 |
gagctc atgcaattttgccttcag
ctcgagttaatacctgtagtgttccgacttg |
succinyl-CoA synthetase
subunitalpha
(point 8) |
903 |
94/30 |
58/30 |
72/60 |
+ |
+ |
+ |
+ |
7 |
catatggtgcaaatcaacaggagaggc
ctcgagtcagatgtcggcggcgatac |
peptidyl-prolyl cis-trans isomeraseA (point 10) |
531 |
94/30 |
57/30 |
72/60 |
+ |
+ |
+ |
+ |
8 |
catatgatgtccattctcgtcaacaag
ctcgagtcagcccttgaggacttcaac |
S-adenosyl-L-
homocysteine hydrolase
(point 9) |
1446 |
94/30 |
58/30 |
72/60 |
+ |
+ |
+ |
+ |
9 |
catatgatggcaaagagtaagtttgaacgtac
ctcgag ttactcgatgatcgacgagacg |
Protein translation elongation
factor EF-TU
(point 2) |
1176 |
94/30 |
58/30 |
72/60 |
+ |
+ |
+ |
+ |