Blast/mBLAST     Samples Sequences Aligned to KEGG+c and/or UNIREF100 (days) analysis Body Region Body site (#) (Millions) BLAST mBLAST BLASTX and mBLASTX (metabolic profiling) Nasal Cavity Anterior_nares 88 141 1,351.3 0.8 Oral Cavity Buccal_mucosa 109 1,344 12,882.9 7.9   Supragingival_plaque 116 6,651 63,741.4 39.3   Tongue_dorsum 125 10,630 101,875.5 62.7 GI Tractb Stool 139 14,472 138,689.7 85.4 Vaginal Tract Posterior_fornix 54 250 2,396.5 1.5 TBLASTX  andmTBALSTX (virus discovery) Nasal Cavity Anterior_nares 88 94 2,122.6 0.3 Oral Cavity Buccal_mucosa 109 527 11,875.0 1.8   Supragingival_plaque 116 3,006 67,747.9 10.4   Tongue_dorsum 125 4,986 112,351.4 17.3 GI Tractb Stool 139 5,615 126,535.3 19.5 Vaginal Tract Posterior_fornix 54 57 1,279.2 0.2 BLASTP and mBLASTP (ORF annotation)   All body site ORFs 631 90 924.8 1.2 aThe timing is based on performance using Large machine (dual socket-quad Nehalem core 48 GB memory); b GI Tract, Gastro Intestinal Tract; c KEGG+, is the combination of the KEGG database and 6 other functional databases.

Table 3: The Human Microbiome Dataset and time required for analysisa.