Manual Machine No injection Dead 103 27.5% 220 27.7% 75 25.0% EGFP(-) 140 37.4% 232 29.2% 225 75.0% EGFP(+) 131 35.0% 343 43.1% - - Total 374   795   300   Injection solution: pXI-EGFP-His (50 ng/μl) and 100 μM (micro M) Rhodamine B

Table 1: Injection efficiency with manual and injection machine.