Manual
Machine
No injection
Dead
103
27.5%
220
27.7%
75
25.0%
EGFP(-)
140
37.4%
232
29.2%
225
75.0%
EGFP(+)
131
35.0%
343
43.1%
-
-
Total
374
795
300
Injection solution: pXI-EGFP-His (50 ng/μl) and 100 μM (micro M) Rhodamine B
Table 1:
Injection efficiency with manual and injection machine.