Mean n-mer coverage Pd n-mer ZCAL Contextual ZBm B.malleiATCC 23744 116.50 0.99985 3.06 -0.73 B.malleiSAVP1 121.36 0.99800 49.93 -0.66 B.malleiNCTC-_10229 116.75 0.99660 79.13 -0.60 B.malleiNCTC_10247 116.13 0.99650 79.13 0.61 B.pseudomallei1106a 97.88 0.94000 1015.70 1.63 B.pseudomallei668 97.65 0.93720 1064.30 1.74 B.pseudomalleiK96243 94.59 0.92850 1191.05 2.09 B.pseudomallei1710b 93.50 0.92400 1201.05 2.25 Burkholderia 383 70.86 0.82600 1770.90 6.12 Burkholderiathailendensis 88.56 0.88030 1839.60 3.99 Burkholderiamultivorans 80.85 0.83960 2055.80 5.59

Table 3: Z values and contextual Z values for Burkholderia mallei strain China 7, which is most closely matched to B. mallei ATCC 23744. Scores against other Burkholderia are shown for context. While the Z value of 3.06 is high, we show that this is due to far greater population structure (“biological noise”) in B. mallei samples as compared to the Bgcalibrant sample, as well as consensus SNPs.