Mean n-mer coverage Pd n-mer ZCAL Contextual ZBp B.pseudomallei 668 98.86 0.99982 2.87 -1.65 B.malleiSAVP1 113.50 0.98680 278.50 -1.54 B.malleiATCC_23744 108.55 0.98550 282.00 -1.52 B.malleiNCTC_10247 108.50 0.98530 287.91 -1.53 B.malleiNCTC_10229 109.04 0.98520 293.79 -1.53 B.pseudomallei1106a 95.55 0.96830 528.05 -1.38 B.pseudomalleiK96243 92.37 0.95630 682.25 -1.28 B.pseudomallei 1710b 91.42 0.95310 755.40 -1.24 Burkholderia 383 70.86 0.82600 1770.90 6.12 Burkholderiathailendensis 88.56 0.88030 1839.60 3.99 Burkholderiamultivorans 80.85 0.83960 2055.80 5.59

Table 4: Z values and contextual Z values for Burkholderia pseudomallei strain 668. As for the B. mallei, the elevated Z value against the reference is attributable to greater n-mer diversity (population structure) as well as additional consensus SNPs compared to the Bg calibration sample.