Locus Repeat Primer sequence (5’–3’) s Ta ( C°) A GenBank Cross-amplification
Ed1 (AC)6(AG)17 F: ACACACACACACAGAGAGAGAG
R: CTGATGTTGCCACGGAGT		 277
265-303		 54 10 JX193598  
Ed2 (AC)6(AG)7 F: ACACACACACACAGAGAGAGAG
R: TCAAAACACAAAAAAACTCA		 171
167-194		 52 9 JX193599  
Ed3 (AC)6(AG)6 F: ACACACACACACAGAGAGAGAG
R: ATACAAATTGAACAAGGGCTTA		 314
312-330		 53 8 JX193600  
Ed4 (AC)6(AG)6 F: ACACACACACACAGAGAGAGAG
R: AGTTTGAGGCTTTATTCAGTTT		 213
211-225		 54 5 JX193601  
Ed5 (AC)6(AG)7 F: ACACACACACACAGAGAGAGAG
R: ACAGGGTTCTTGCTATTTCA		 187
185-218		 53 7 JX193602  
Ed6 (AC)6(AG)9 F: ACACACACACACAGAGAGAGAG
R: GCCACCAATCCTTGAACCT		 175
169-212		 54 9 JX193603 a, b
Ed7 (AC)6(AG)7 F: ACACACACACACAGAGAGAGAG
R: TGTCATTGATGGGAAAAACT		 291
289-334		 53 10 JX193604  
Ed8 (AC)6(AG)11 F: ACACACACACACAGAGAGAGAG
R: AAATGTCATAATCAAAAAGCAG		 134
124-154		 51 9 JX193605  
Ed9 (AC)6(AG)6 F: ACACACACACACAGAGAGAGAG
R: TGATTCTTGATGTCCTTCTATT		 179
177-199		 54 5 JX193606 a
Ed10 (AC)6(AG)9 F: ACACACACACACAGAGAGAGAG
R: GCTTTTGAGGGCTATTGATG		 216
208-232 		54 8 JX193607  
Ed11 (AC)6(AG)11 F: ACACACACACACAGAGAGAGAG
R: CATCACCTTTTTCCCTATCA		 402
394-418		 53 5 JX193608 a, b
Ed12 (AC)6(AG)6 F: ACACACACACACAGAGAGAGAG
R: TCGGGAATGAAAAAAAATAG		 159
157-204		 53 5 JX193609 a, b
Ed13 (AC)6(AG)6
CG(AG)5		 F: ACACACACACACAGAGAGAGAG
R: GGGAGATAGAGATAGAGACG		 184
174-199		 55 4 JX193610  
Ed14 (AC)6(AG)6 F: ACACACACACACAGAGAGAGAG
R: ATTTCATTTGGTGGGCTTT		 284
284-308		 55 4 JX193611  
Ed15 (AC)6(AG)8 F: ACACACACACACAGAGAGAGAG
R: ATCCTTTTTAGATTTCGTTTTA		 195
190-223		 54 5 JX193612  
Ed16 (AC)6(AG)11 F: ACACACACACACAGAGAGAGAG
R:TACCACATAAACAAACCATT		 386
376-411		 54 9 JX193613  
Ed17 (AC)6(AG)12 F: ACACACACACACAGAGAGAGAG
R:TTATCAAAAAATCAACAAAT		 291
280-322		 53 11 JX193614  
Ed18 (AC)6(AG)6
AC(AG)3		 F: ACACACACACACAGAGAGAGAG
R:CGGTTATGCCACGGACTT		 277
271-298		 56 4 JX193615  
Notes: The a and b represent the primers that can successfully amplify in E. sylvestris and E. japonicus
Table 1: Characteristics of 18 compound microsatellite loci developed for E. decipiens. Shown for each locus are the locus name, the forward (F) and reverse (R) primer sequence, the optimized annealing temperature (Ta), allele size ranges, the total number of alleles per locus (A) and the GenBank accession number. Size ranges and the total number of alleles include all values detected within three E. decipiens populations used in this study (Table 2).