Locus Population JG (11) Population LHN(12) Population TB(12) A Ho He PIC A Ho He PIC A Ho He PIC Ed1** 9 0.818 0.905 0.849 8 0.583 0.848 0.789 8 0.750 0.859 0.800 Ed2 * 6 0.727 0.823 0.753 8 0.833 0.899 0.845 8 0.750 0.884 0.828 Ed3 n.s 5 0.818 0.797 0.720 6 0.917 0.855 0.794 7 0.667 0.837 0.777 Ed4 n.s 5 0.909 0.801 0.726 5 0.750 0.815 0.745 5 0.750 0.797 0.723 Ed5 n.s 7 0.727 0.874 0.813 5 0.833 0.815 0.746 6 0.750 0.797 0.731 Ed6* 7 0.818 0.831 0.765 7 0.750 0.804 0.738 8 0.750 0.855 0.797 Ed7* 7 0.818 0.866 0.803 7 0.833 0.884 0.828 7 0.667 0.833 0.770 Ed8 n.s 7 0.818 0.857 0.794 7 0.750 0.870 0.811 7 0.750 0.804 0.740 Ed9 n.s 5 0.818 0.827 0.756 5 0.750 0.812 0.741 5 0.750 0.819 0.750 Ed10** 7 0.818 0.840 0.773 6 0.667 0.841 0.778 7 0.583 0.866 0.808 Ed11 n.s 5 0.818 0.779 0.700 5 0.667 0.815 0.745 5 0.750 0.786 0.716 Ed12 n.s 5 0.818 0.766 0.687 5 0.583 0.808 0.737 5 0.750 0.830 0.762 Ed13 n.s 4 0.818 0.723 0.627 4 0.750 0.736 0.654 4 0.833 0.685 0.605 Ed14 n.s 4 0.818 0.775 0.691 4 0.750 0.764 0.683 4 0.833 0.764 0.683 Ed15 n.s 4 0.818 0.775 0.691 4 0.750 0.757 0.677 5 0.750 0.808 0.737 Ed16 n.s 9 0.818 0.853 0.791 6 0.750 0.851 0.790 6 0.750 0.819 0.753 Ed17** 10 0.818 0.909 0.854 7 0.833 0.884 0.828 9 0.667 0.902 0.850 Ed18 n.s 4 0.818 0.740 0.651 4 0.833 0.761 0.678 4 0.750 0.772 0.691 Notes: *, ** and ***, significant departures from Hardy–Weinberg equilibrium at P<0.05, P<0.01, P<0.001, respectively. n.s.= not significant.

Table 2: Results of initial primer screening in three populations of E decipiens. Shown are locus name, the number of alleles per locus (A), mean values of observed (Ho) and expected (He) heterozygosity, and polymorphism information content (PIC). The sample size for each population is shown in parentheses.