| Gene |
Primer pair sequence |
Annealing temperature(cycle) |
Product size |
| IFN-γ |
| MF: 5'- AAGAGTTAATATTTTATTAGGGCGA-3' |
52°C (35) |
151 bp |
| MR: 5'-TAAACTCCTTAAATCCTTTAACGAT-3' |
| UF: 5'-TGAAGAGTTAATATTTTATTAGGGTGA -3' |
52°C (35) |
154 bp |
| UR: 5'-TAAACTCCTTAAATCCTTTAACAAT -3' |
| SOCS1 |
| MF: 5'-TTGTTCGGAGGTCGGATTT-3' |
65°C (35) |
218 bp |
| MR: 5'-ACTAAAACGCTACGAAACCG-3' |
| UF: 5'-TTTTTTGGTGTTGTTTGGAGGTTGGATTTT-3' |
60°C (35) |
243 bp |
| UR:5'-AAAACAAAACAATAAACTAAAACACTACAAAA
CCA-3' |
| SOCS3 |
| MF: 5'-GGAGATTTTAGGTTTTCGGAATATTTC -3' |
60°C (30) |
142 bp |
| MR: 5'- CCCCCGAAACTACCTAAACGCCG -3' |
| UF: 5'-GTTGGAGATTTTAGGTTTTTGGAATATTTT-3' |
60°C (30) |
151 bp |
| UR: 5'-AAACCCCCAAAACTACCTAAACACCA -3' |
M: Methylated; U: Unmethylated; F: Forward; R: Reverse.