microRNA Target accession Target description Target regulation by brassinosteroid ath-miR156h AT3G57920 Squamosa promoter binding protein-like 15 up ath-miR822 AT5G02330 Cysteine/Histidine-rich C1 domain family protein up ath-miR824 AT3G12470 Polynucleotidyltransferase up ath-miR843 At3g13840 GRAS family transcription factor up ath-miR854a AT5G05090 Homeodomain-like superfamily protein up ath-miR391 AT3G02380 CONSTANS-like 2 protein down MicroRNA targets were predicted using psRNAtarget program (http://plantgrn.noble.org/psRNATarget/) with default parameter settings Brassinosteroid-responsive genes were determined by the AtGenExpress database (Arabidopsis eFP Browser, http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Hormone)

Table 2: Predicted microRNA targets that are brassinosteroid-responsive genes.