Target IVS |
Primer pair (5’-> 3’) |
Amplicon size (bp) |
AT1 (°C) |
Host specificity |
| Anaerovibrio_1 |
Ana_F1: gtgaggcccccgaaaacag
Ana_R1: tccatttctaacacccagtctct |
76 |
45 to 65 gradient |
NSA2 |
| Anaerovibrio_2 |
Ana_F2: gcttgatagacagcgtcgtg
Ana_R2: atccattcctaacacccagtcac |
91 |
45 to 65
gradient |
NSA |
| Anaerovibrio_3 |
Ana_F3: tgcaaagcaaaaccggctac
Ana_R3: agcatccacgctaacactcaa |
82 |
59 |
Beef cattle and pig |
| Bacteroides_1 |
Bac_F1: tcattcgagagcagcggtaaa
Bac_R1: ttcctcgctgcatgtgttgt |
83 |
59 |
Beef cattle and pig |
| Faecalibacterium_1 |
Fae_F1: tccgagccttttgcagcgtca
Fae_R1: gccactaagcgatttttctactccc |
104 |
55 |
Chicken and turkey |
| Faecalibacterium_2 |
Fae_F2: actccgagttcttcgcgggt
Fae_R2: tcccagctctctgctccacct |
120 |
55 |
Chicken and turkey |
| Faecalibacterium_3 |
Fae_F3: actccgggttctttgcttggct
Fae_R3: actcccagctctccgctcca |
107 |
45 to 65
gradient |
NSA |
| Faecalibacterium_4 |
Fae_F4: ttgcgagtcagccggaagcg
Fae_R4: tgccctttgtgtcagccttactgg |
75 |
60 |
Beef cattle and pig |
| Mitsuokella_1 |
Mit_F1: gagcggactgatgagaggc
Mit_R1: catcactcagccttaaagttaagcact |
81 |
62 |
Horse, human, and pig |
| Peptostreptococcus_1 |
Pep_F1: agggcttactcggaattgag
Pep_R1: cggcacttaatagtgcgtgt |
95 |
55 |
Human |
| Phascolarctobacterium_1 |
Pha_F1: cggagcaaattttattacgctctt
Pha_R1: cgccactaagagtatccaacac |
93 |
45 to 65
gradient |
NSA |
| Subdoligranulum_1 |
Sub_F1: gctgaaaggtgagcgtcaatc
Sub_R1: attttcttcactcccagccc |
91 |
55 |
Chicken and turkey |
| Subdoligranulum_2 |
Sub_F2: tgcacgcttccggcaagc
Sub_R2: actcccaatcctctgcc |
84 |
55 |
Pig |
