A) Correlations of log2 expression levels (RPM) in two individual biological replicates of RAW264 for each condition are shown. (B) Metagene analyses near the start codon of analyzed genes are shown. 5’ ends of sequenced reads were aligned and read counts were divided by the mean read counts of the first 1-kb coding region in each condition. RPM: Reads Per Million.
Figure 1: Both mRNA-Seq and Ribo-Seq showed strong reproducibility and validity.