Figure 1: Western blotting analysis of human AD and normal samples and workflow for identification of Cdc37-interacting proteins by mass spectrometry. (a) Lysates from AD (AD10, AD12, AD37) and normal (NAD11, NAD29, NAD41) brain cortex samples were analyzed by Western blotting for expression of hyperphosphorylated tau, total tau, Cdc37, Hsp90 and GAPDH. (b) Schematic of the experimental workflow employed to 0identify Cdc37 interacting proteins using mass spectrometry. Immunoprecipitation of Cdc37 was confirmed by Western blotting.