Bacterial proteins |
Bacterial self - antigens |
Homologous Human proteins |
Functions inspermatogenesis/male fertility |
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RNLRKKLD |
SPNR |
microtubule - associated RNA - binding protein localizes |
DNA response regulator protein |
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to the manchette in developing spermatids; |
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normal spermatogenesis and sperm function |
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KDGQEA |
TOPAZ1 |
Abundantly expressed in gonads during germ cell |
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meiosis;involved in germ cell development and gametogenesis |
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QARVKNEE |
SA |
Involved in fertilization and P36 like sperm antigen |
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is involved in male infertility |
Laminin binding protein |
KAFKK |
MAPO2 |
Helps in the induction of apoptosis of cells with |
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O6 - methylguanine lesion |
Hyaluronate lyase |
EERNL |
TPAP |
cytoplasmic poly(A) polymerase expressed in round |
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spermatids; role in spermiogenesis |
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VATKPL |
TPABP |
mRNA stability and translation initiation in testis |
Streptococcal peptides showing resemblance with at least 5 contiguous amino acids of human peptides without any gaps and mismatcheswere considered as self - peptides. Three self - peptides with amino acid sequence RNLRKKLD, KDGQEA and QARVKNEE representing human proteins namely Spermatid perinuclear RNA binding protein (SPNR), Testis - and ovary - specific PAZ domain - containing protein (TOPAZ1) and Sperm antigen protein (SA) were obtained from DNA binding response regulator protein. One self - peptide was obtained from Laminin binding protein with amino acid sequence KAFKK which represents human Motile sperm domain containing protein (MAPO2). Finally, two self - peptides EERNL and VATKPL representing human Testis - specific poly(A) polymerase protein (TPAP) and Testis - specific poly(A) binding protein (TPABP)were obtained from Hyaluronate lyase protein, respectively. Each of these self - peptides serves to play a major role in spermatogenesis and male fertility function. Out of six putative self - peptides only three peptides of functional importance can be selected for the preparation of immunocontraception/vaccine construct namely RNLRKKLD (SPNR protein), QARVKNEE (Sperm antigen) and KAFKK (MAPO2). These self - peptides only affect the sperm functions and not the spermatogenesis mechanism. Thus, they can be targeted for a temporary period and can be reversed anytime. |