Figure 4: Hemagglutination-inhibition test. Well A12-negative control (no IBs, no fetuin); line A (1-11) - negative control (no fetuin); lines B-H-inhibition of hemagglutination by fetuin. Suspension of IBs was diluted in ratio 1:8 and resulted suspension was 1,3-fold serial diluted in 15 μl PBS from column to column. Fetuin (66,67 mg/ml) was 1,5-fold diluted in 10 μl PBS (final concentration 8,89 mg/ml in well) from line H to line B. Described mixture was then titrated with 50 μl of washed erythrocytes. Wells in frames correspond to linear regressions depicted on diagram.