Figure 2: Degradation of HS in the BM by NMuMG cell lysate. BM-like matrices that had been metabolically labeled with [35S]-sulfate were incubated with lysates from untreated (open circles) and TGF-ß3-treated cells (closed circles), mouse recombinant heparanase (solid line), or lysis buffer (broken line). Supernatants containing released HS were applied to a Sephacryl S-400 gel filtration column and counted.