Figure 5: Influence of heparanase overexpression on TGF-b3-induced EMT of NMuMG cells. NMuMG cells that had been transfected with heparanase gene or empty vector were cultured with 1 ng/ml TGF-β3 for 48 h. Heparanase content in cell lysates (A), cell shape after the incubation (B, scale bar: 50 μm), mRNA expression of E-cadherin (C) and N-cadherin (D) are indicated.