| |
SL |
MRM transition ion pair (m/z) |
Calibration curve |
Correlation coefficient (r2) |
LOD (S/N=5) |
| Cer |
C16
C18
C24 |
538.6>264.3
566.6>264.3
650.7>264.3 |
y=0.4746x-0.0068
y=0.5681x-0.0117
y=0.3270x-0.0040 |
0.9911
0.9905
0.9917 |
1 pg
1 pg
1 pg |
| GC |
C16
C18
C24-1 |
700.7>264.4
728.8>264.4
810.8>264.3 |
y=0.1856x-0.0016
y=0.2757x-0.0016
y=0.3202x-0.0029 |
0.9950
0.9982
0.9941 |
1 pg
1 pg
1 pg |
| LacCer |
C16
C24 |
862.7>264.3
974.8>264.3 |
y=1.7887x+0.0055
y=1.3099x+0.0027 |
0.9986
0.9991 |
1 pg
10 pg |
| Sulfatide |
C16
C24 |
778.7>97.0
890.6>97.0 |
y=0.1252x+0.0100
y=0.4184x+0.0361 |
0.9942
0.9917 |
50 pg
50 pg |
| Curves for GC and GalCer on SP-LC-MS/MS |
| GC |
C16
C18
C24-1 |
700.7>264.4
728.8>264.4
810.8>264.3 |
y=0.2163x-0.0013
y=0.2370x+0.0007
y=0.2243x-0.0018 |
0.9997
0.9999
0.9997 |
25 pg
25 pg
25 pg |
| GalCer |
C16
C24-1 |
700.7>264.4
810.8>264.3 |
y=0.2275x-0.0071
y=0.3142x-0.0083 |
0.9997
0.9996 |
25 pg
25 pg |
