Figure 1: Gene expressions by quantitative PCR analysis and whole-mount in situ hybridization for zPOMGnT1. (A, B) Quantitative PCR analysis of zPOMGnT1 mRNA in adult tissues (A) and during early developmental stages (B). zPOMGnT1 was detected in all tissues predominantly in ovary and throughout early developmental stages. Internal organs in female zebrafish showed higher level of expression than in male ones. zβ-actin2 and zCox1 were used as internal controls for quantitative PCR in adult tissues and developmental stages, respectively. B, brain; E, eye; H, heart; M, muscle; T, testis; O, ovary; LM, liver (male); LF, liver (female); IM, intestine (male); IF, intestine (female); KM, kidney (male); KF, kidney (female); SM, spleen (male); SF, spleen (female). All reactions were performed in triplicate, and average values ± SD are shown. (C) Whole-mount in situ hybridization for zPOMGnT1 during early developmental stages. zPOMGnT1 mRNA was expressed ubiquitously throughout developmental stage. Scale bar = 200 μm.