Figure 3: Detection of the recombinant zPOMGnT1 and its enzymatic activity. (A) Western blot analysis of zPOMGnT1 fused to His-tag expressed in HEK293T cells. An arrowhead indicates the expressed zPOMGnT1. (B) Assay of POMGnT1 activity. Specific activity was determined using mannosyl nanopeptide as an acceptor.