Concentration (mM) Normal Moderate Severe Wild Type - 90 (98.9%) 1 (1.1%) 0 (0%) Control MO 1.00 114 (95.8%) 5 (4.2%) 0 (0%) zPOMGnT1MO 1.00 38 (58.5%) 1 (1.5%) 26 (40.0%)   0.50 60 (71.4%) 14 (16.7%) 10 (11.9%)   0.25 82 (95.3%) 3 (3.5%) 1 (1.2%)

Table 1: Quantification of zPOMGnT1 morphant phenotypes at 96 hpf. The number of embryos observed for each phenotypic class is shown and also presented as a percentage of the total number of embryos studied for each morpholino. Moderate phenotype showed curved tail and small eyes and severe phenotype showed twisted tail, small eyes and edematous pericardium.