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Figure 1: ELISA analysis using polySia-specific antibodies. The plastic wells were coated with various concentrations of PEB and PAB samples (0-50 µg/ ml as BSA) and incubated at 37°Cfor 2 hours. After blocking with 1% BSA, anti-polySia antibodies, 12E3 (1 µg/ml) specific to (Neu5Ac)5 or greater with non-reducing terminal end and 735 (0.8 µg/ml) specific to (Neu5Ac)11 or greater were incubated. After washing, peroxidase conjugated anti-mouse IgG+IgM were incubated and color development was performed as described in Materials and Methods. |