| Peak no.in Figure. 3 |
Time (min) |
Site |
Fragment |
Amino acid sequencea |
Carbohydrate |
Charge state |
Observed m/z |
Measured mass |
Monoisotopic mass |
| 1 |
3.8 |
185 |
185-193 |
SPPGADPR |
[Hex][HexNAc] |
2+ |
581.24 |
1160.46 |
1160.52 |
| 2 |
5.7 |
74 |
70-75 |
RCQTNL |
[Fuc][GlcNAc]2[Man]3 |
2+ |
915.75 |
1829.50 |
1829.74 |
| 3 |
8.0 |
45 |
43-48 |
FHNESL |
[GlcNAc] |
2+ |
475.18 |
948.36 |
948.42 |
| 4 |
10.4 |
38 |
34-42 |
SPEDNSTQW |
[Fuc][GlcNAc]2[Man]3 |
2+ |
1051.28 |
2100.56 |
2100.80 |
| 5 |
11.2 |
45 |
43-52 |
FHNESLISSQ |
[GlcNAc] |
2+ |
682.76 |
1363.51 |
1363.63 |
| 6 |
11.8 |
74 |
70-78 |
RCQTNLSTL |
[Fuc][GlcNAc]2[Man]3 |
2+ |
1066.29 |
2130.58 |
2130.90 |
| 7 |
12.5 |
64 |
58-69 |
IDAATVNDSGEY |
[Fuc][GlcNAc]2[Man]3 |
2+ |
1146.84 |
2291.68 |
2291.92 |
| 8 |
13.1 |
64 |
58-69 |
IDAATVNDSGEY |
[GlcNAc] |
2+ |
729.24 |
1456.48 |
1456.62 |
| 9 |
13.5 |
180 |
177-184 |
AVSTISSF |
[HexNAc]2[Hex]2 |
2+ |
771.27 |
1540.54 |
1540.68 |
| 10 |
14.2 |
45 |
43-56 |
FHNESLISSQASSY |
[Fuc][GlcNAc]2[Man]3[GlcNAc]2[Man]3 |
2+2+ |
1304.39
1231.34 |
2606.78
2460.68 |
2607.09
2461.03 |
| 11 |
15.1 |
180 |
177-184 |
AVSTISSF |
[HexNAc][Hex] |
2+ |
588.74 |
1175.48 |
1175.54 |
| 12 |
17.1 |
64 |
57-69 |
FIDAATVNDSGEY |
[Fuc][GlcNAc]2[Man]3 |
2+ |
1220.34 |
2438.68 |
2438.99 |
| 13 |
18.0 |
64 |
57-69 |
FIDAATVNDSGEY |
[GlcNAc] |
2+ |
802.76 |
1603.52 |
1603.69 |
| 14 |
20.3 |
162,169 |
158-176 |
VGSKNVSSETVNITITQGL |
[Fuc][GlcNAc]2[Man]3[Fuc] [GlcNAc]2[Man]3 |
3+ |
1341.76 |
4022.28 |
4022.78 |
aC:Carboxymethyl cysteine |
