| DNA fragments |
sense primer (5' → 3') |
antisense primer (5' → 3') |
| zNMT1 full (for pET50b) |
ATGCGTCGACGCGGATGAGAATGAGAC |
ATAGCGGCCGCCTGCAGAACCAATCC |
| zNMT1 full |
CGAGAATTCCACCATGGCGGATGAG |
ATAGCGGCCGCCTGCAGAACCAATCC |
| zNMT1 deltaC1 |
CGAGAATTCCACCATGGCGGATGAG |
ACGTAGCGGCCGCTCAGAAGGAATATGCCGCC |
| zNMT1 deltaC2 |
CGAGAATTCCACCATGGCGGATGAG |
ACGTAGCGGCCGCTCAAGTGTACACAGCCTGAAAG |
| zNMT1 deltaN1 |
GCTATGAATTCGAGGAGGCCACACGTC |
ATAGCGGCCGCCTGCAGAACCAATCC |
| zNMT1 deltaN2 |
GCTATGAATTCGTGGAGATTAACTTTCTGTG |
ATAGCGGCCGCCTGCAGAACCAATCC |
| zNMT2 full |
ATGCGAATTCGCGGAGGACAGCGAGTC |
ACGTAGCGGCCGCTTACTGTAAAACAAGGCCAAC |
| zNMT2 deltaC1 |
ATGCGAATTCGCGGAGGACAGCGAGTC |
ACGTAGCGGCCGCTCAAGTGTCAATGATGTTC |
| zNMT2 deltaC2 |
ATGCGAATTCGCGGAGGACAGCGAGTC |
ACGTAGCGGCCGCTCAACCAGCAGTATACAC |
| zNMT2 deltaN1 |
GCTATGAATTCGAGCTCCTGTCCAC |
ACGTAGCGGCCGCTTACTGTAAAACAAGGCCAAC |
| zNMT2 deltaN2 |
GCTATGAATTCGTGGAGATTAACTTCTTG |
ACGTAGCGGCCGCTTACTGTAAAACAAGGCCAAC |
