Figure 2: Relative abundance of hFSH21 in pituitaries of 12 women as a function of age. A. Collage of representative Western blots from each individual. Lanes 1-12 were included in the correlation analysis. Lanes a-c were excluded due to steroid treatment, which appeared to increase the abundance of the hFSHβ21 band in 2 of 3 cases. B. Plot of average (±Std. Dev.) relative abundance of the hFSHβ21 band determined by Western blots for three or four 1-μg samples of each hFSH preparation. A highly significant negative correlation was associated with increasing age.