Figure 3: Neuraminidase-digested oligosaccharides analyzed by nano-ESI mass spectrometry. Aliquots of samples analyzed in Fig. 2 were digested with Arthrobacter ureafaciens sialidase prior to analysis. Structures of the more abundant neutral core glycans (Table 1) are shown in each panel. A. Desialylated glycans from purified pituitary hFSH. B. Desialylated glycans from purified urinary hFSH. C. Desialylated glycans from recombinant GH3-hFSH. D. Desialylated glycans from hFSH24. E. Desialylated glycans from hFSH21. F. Desialylated glycans from hypo-glycosylated hFSH21/18. Note the expanded scale indicated by the dashed line.