Neutral compounds Acidic compounds
m/za,b Comp.c Sampled,e,f Structuree m/z Acid groupsg Ionh Sampled,e,f
H N F P U G F24 F21 F21/18 Si SO3H P U G F24 F21 F21/18
1007.3 3 2 0 0.11 - - 0.37 0.64 5.10 1 - - - - - - - - - -
1153.4 3 2 1 0.18 - - 0.47 0.54 4.82 2 - - - - - - - - - -
1169.3 4 2 0 - - - 0.17 - 0.74 3 - - - - - - - - - -
1210.4 3 3 0 - - - 0.12 0.15 1.95 4,5 - - - - - - - - - -
1331.4 5 2 0 - - - 0.52 0.29 11.67 6 - - - - - - - - - -
1356.5 3 3 1 - - 0.91 0.29 - 2.41 7,8 - - - - - - -   - -
1372.5 4 3 0 - - - 0.49 0.47 0.96 9,10 1565.5 1 0 1 0.76 0.23 - 0.58 1.33 1.74
1413.5 3 4 0 - - - 0.33 0.28 1.69 11,12 1395.4 0 1 1 - - - 1.56 1.04 1.13
1606.6 1 0 1 0.21 - - 0.46 0.66 0.77
1493.5 6 2 0 - - - 0.11 0.12 11.20 13 - - - - - - - - - -
1518.5 4 3 1 - 1.10 - 0.34 0.26 0.95 14,15 1711.6 1 0 1 0.62 0.17 - 0.38 0.74 0.97
1559.5 3 4 1 -   - 0.07 0.12 2.84 16,17 1541.5 0 1 1 - - - 0.44 0.74 0.37
1575.5 4 4 0 - 0.11 0.54 0.22 0.22 0.64 18,19 1557.5 0 1 1 - - - 0.03 0.85 -
1768.6 1 0 1 - - 0.66 0.30 0.46 0.54
1616.6 3 5 0 - - 2.48 0.10 - 0.85 20,21 1598.5 0 1 1 - - - 0.27 0.29 0.41
1809.7 1 0 1 - - 0.11 0.12 0.15 0.32
1655.5 7 2 0 - - - 0.09 0.10 7.31 22,23,24 - - - - - - - - - -
1721.6 4 4 1 - - 0.90 0.26 0.30 0.78 25,26 1703.5 0 1 1 - - - 0.11 0.14 -
1914.7 1 0 1 0.26 0.09 - 0.20 0.27 0.47
1737.6 5 4 0 - 0.20 0.28 0.83 0.10 0.30 27 1004.8 1 1 2 0.25 0.41 - 0.32 0.29 -
1110.4 2 0 2 9.25 8.82 10.61 4.36 4.09 1.86
1719.5 0 1 1 - - - 0.27 0.34 -
1930.7 1 0 1 0.35 1.56 0.88 1.85 0.63 0.32
2221.8 2 0 1 0.12 0.57 0.37 0.13 0.14 -
1762.6 3 5 1 - - - - - 0.82 28,29,30 1744.6 0 1 1 - - - 0.22 0.26 0.13
1778.6 4 5 0 - 0.12 2.27 0.65 0.24 0.59 31,32,33 919.8 0 2 2 - - - 0.26 0.22 -
1025.3 1 1 2 2.05- 0.82 - 2.81 3.97 2.34
1130.9 2 0 2 6.57 3.97 8.06 3.78 6.19 2.86
1760.6 0 1 1 0.18 0.16 0.67 1.30 0.78 0.33
1971.7 1 0 1 0.48 0.67 2.47 1.50 1.07 0.83
2262.8 2 0 1 0.05 0.09 - 0.08 0.11 -
1817.6 8 2 0 - - - 0.08 0.13 11.50 34 - - - - - - - - - -
1819.6 3 6 0 - - - - - - 35,36 940.3 0 2 2 0.23 0.06 - 2.80 2.15 1.14
1045.8 1 1 2 0.29 0.14 - 0.42 0.49 0.28
1801.6 0 1 1 - - - 0.04 - -
2012.7 1 - 1 - - - - - 0.08
1883.6 5 4 1 -   0.22 -   0.54 0.10 0.38 37,38 1077.8 1 1 2 - 0.39 - 0.34 0.29 -
1183.4 2 0 2 7.66 8.61 12.96 2.99 3.77 1.16
1865.6 0 1 1 - - - 0.10 0.02 -
2076.7 1 0 1 0.34 1.35 1.80 1.03 0.46 0.24
2367.8 2 0 1 0.05 0.14 - 0.12 0.06 -
1924.6 4 5 1 - 0.06 2.18 0.34 0.13 0.38 39,40,41
42,43,44
992.8 0 2 2 - - - T - -
1098.4 1 1 2 1.08 0.45 - 3.27 4.94 1.59
1203.9 2 0 2 0.62 0.47 2.44 0.93 1.24  
1906.6 0 1 1 T 0.08 - 1.05 0.63 0.21
2117.8 1 0 1 T 0.18 2.39 - 0.28 0.29
2197.7 1 1 1 - - - - 0.12 -
1940.7 5 5 0 - 0.11 0.49 0.35 0.07 0.26 45 1211.9 2 0 2 4.44 4.03 4.90 1.79 2.90 0.74
2133.8 1 0 1 0.19 0.67 1.44 0.60 0.35 0.25
2424.9 2 0 1 0.04 0.07 0.30 - - -
1965.7 3 6 1 - - - - - 0.27 46,47 1013.3 0 2 2 0.16 - - 5.46 7.23 1.14
1053.3 0 3 2 - - - 0.15 0.13 -
1118.9 1 1 2 - - - 0.46 0.49 -
1947.7 0 1 1 - -   0.15 0.11 0.09
1979.6 9 2 0 - - - - - 4.85 48 - - - - - - - - - -
1981.7 4 6 0 - - - - - - 49 1963.7 0 1 1 - - - 0.03 - -
2174.8 1 0 1 - - - - - 0.09
2029.7 5 4 2 - - - - 0.03 0.04 50,51 1256.4 2 0 2 0.28 - - 0.32 0.18 -
2222.8 1 0 1 - - 0.37 - - -
2070.7 4 5 2 - - 0.87 0.09 0.05 0.12 52,53 2052.7 0 1 1 - - - 0.23 0.34 0.18
2086.7 5 5 1 - 0.12 0.66 0.11 0.07 0.22 54,55,56 1179.4 1 1 2 - - - 0.10 0.07 -
1285.0 2 0 2 4.72 2.75 4.51 2.03 3.27 0.94
2279.8 1 0 1 0.16 0.37 1.16 0.50 0.25 0.13
2102.7 6 5 0 - 0.05 - 0.22 - 0.08 57,58 1292.9 2 0 2 4.44 8.15 3.01 5.27 2.69 0.29
1438.5 3 0 2 6.48- 5.08 4.36 1.78 2.72  
2295.8 1 0 1 0.03 0.36 - 0.58 0.10 0.03
2586.8 2 0 1 T 0.07 - 0.05 - -
2111.7 3 6 2 - - - - - - 59 2093.7 0 1 1 - - - 0.16 0.16 -
2127.7 4 6 1 - - - - - 0.09 60,61 1199.9 1 1 2 - - - 0.11 0.24 -
1313.5 2 0 2 - - - 2.58 - -
1458.9 3 0 2 - - - 0.49 - -
2117.7 1 0 1 0.50 - - - - -
2109.7 0 1 1 - - - 0.04 0.02 -
2117.7 1 0 1 - - - 0.40 - -
2320.8 1 0 1 - - - - - 0.02
2336.8 1 0 1 - - - 0.30 - -
2143.7     5     6     0     -     -     -     0.14     0.02     0.12     62,63,64
65,66,67
68  
1207.9 1 1 2 0.87 0.53   1.51 0.99 -
1313.5 2 0 2 3.12 1.68 2.15 - 3.18 0.49
1353.4 2 1 2 0.84 - - 0.85 0.77 -
1459.0 3 0 2 1.42 0.60 - - 1.28 -
2125.7 0 1 1 - - - 0.17 0.05 -
2336.8 1 0 1 0.05 0.08 - - 0.12 0.03
2184.7 4 7 0 - - - - - - - 1122.8 0 2 2 - - - 0.35 0.17 -
1228.4 1 1 2 - - - 0.63 - -
2232.8 5 5 2 - - 0.50 - - 0.05 69,70 - - - - - - - - - -
2248.8 6 5 1 - 0.05 - 0.16 - 0.07 71,72,73
74
1260.4 1 1 2 0.33 0.35 - 0.39 0.16 -
1366.0 2 0 2 4.39 6.52 4.33 4.52 2.59 0.37
1406.0 2 1 2 -- 0.29 - 0.06 0.04 -
1511.5 3 0 2 4.45 3.06 6.23 1.55 1.86 -
2441.9 1 0 1 0.03 0.28 - 0.36 0.08 0.03
2289.8 5 6 1 - - - 0.03 - 0.06 75,76,77
78
1175.2 0 2 2 - - - 0.17 0.23 -
1280.9 1 1 2 - 0.36 - 1.80 1.90 -
1386.5 2 0 2 0.75 0.47 1.93 0.74 0.86 -
1426.4 2 1 2 - - - 0.81 1.17 -
1532.0 3 0 2 - - - 0.05 0.25 -
2271.7 0 1 1 - - - 0.16 0.06 -
2482.9 1 0 1 0.01 0.04 - 0.08 0.04 0.02
2305.8 6 6 0 - 0.03 - 0.12 - 0.08 79,80 1394.5 2 0 2 3.76 4.74 1.43 2.80 1.79 -
1540.0 3 0 2 5.48 2.61 1.06 0.84 1.32 -
2498.9 1 0 1 0.02 0.16 - 0.22 0.05 0.02
2330.8 4 7 1 - - - - - - 81 1195.9 0 2 2 - - - 0.41 0.39 -
1305.1 1 1 2 - - - 0.22 0.22 -
1228.4 1 1 2 - - - - 0.27 -
2394.8 6 5 2 - - - - - - 82 1439.0 2 0 2 - - 1.72 - - -
2587.9 1 0 1 0.01 - - 0.03 - -
2451.9 6 6 1 - - - 0.10 - 0.05 83,84,85 1467.5 2 0 2 2.81 2.47 1.90 2.40 2.48 0.27
1613.0 3 0 2 2.51 0.98 0.84 0.70 1.84 -
2644.9 1 0 1 0.01 0.07 - 0.12 0.04 0.01
2467.8 7 6 0 - - - 0.03 - - 86,87 1475.5 2 0 2 0.50 5.10 - 1.64 0.91 -
1621.1 3 0 2 1.18 1.05 0.78 1.18 1.07 -
1766.6 4 0 2 0.57 0.23 - 0.29 0.37 -
2660.9 1 0 1 - - - 0.06 - -
2508.9 6 7 0 - - - - - - 88,89 1390.5 1 1 2 - - - 0.39 0.36 -
1496.0 2 0 2 - - - 0.47 0.69 -
1536.0 2 1 2 0.18 - - 0.40 0.44 -
1641.6 3 0 2 0.31 - - 0.16 0.65 -
2549.9 5 8 0 - - - - - - 90 1305.4 0 2 2 - - - 0.18 0.14 -
2613.9 7 6 1 - - - - - 0.02 91,92,93 1548.5 2 0 2 1.30 8.44 - 2.35 0.92 -
1588.5 2 1 2 - 0.16 - 0.05 - -
1694.1 3 0 2 2.72 2.84 1.31 1.29 0.95 -
1839.6 4 0 2 2.57 0.76 0.51 0.26 0.30 -
1985.2 5 0 2 0.23 - - - - -
2807.0 1 0 1 - - - 0.04 0.01 -
2654.9 6 7 1 - - - - - - - 1463.5 1 1 2 - - - 0.55 0.59 -
1569.1 2 0 2 0.23 - 0.59 0.09 0.11 -
1609.0 2 1 2 - - - 0.41 0.42 -
2670.9 7 7 0 - - - - - - 94,95 1577.1 2 0 2 0.37 0.07 - 0.09 0.18 -
1722.6 3 0 2 0.71 0.36 0.71 0.10 0.24 -
1868.2 4 0 2 0.27 - - - 0.07 -
2817.0 7 7 1 - - - - - - 96 1650.1 2 0 2 0.58 0.73 - 0.79 0.69 -
1795.6 3 0 2 1.06 0.50 - 0.28 0.59 -
1941.2 4 0 2 0.81 - -   0.14 -
2833.0 8 7 0 - - - - - - 97 1658.1 2 0 2 0.21 0.09 - 0.12 - -
1803.6 3 0 2 0.21 0.13 - 0.15 0.19 -
1949.2 4 0 2 0.11 - - - - -
2979.1 8 7 1 - - - - - - 98 1731.1 2 0 2 0.20 0.30 - 0.21 0.16 -
1876.7 3 0 2 0.24 0.33 - 0.14 0.14 -
2022.2 4 0 2 0.58 0.29 - - 0.14 -
2167.7 5 0 2 0.10 - - - - -
3020.0 7 8 1 - - - - - - - 1791.6 2 1 2 - - - - 0.08 -
3036.0 8 8 0 - - - - - - 99 1839.6 2 2 2 - - - - 0.30 -
3182.1 8 8 1 - - - - - - - 1832.7 2 0 2 0.12 0.05 - 0.09 - -
1978.2 3 0 2 0.21 - - - - -
3262.1 8 8 1 - - - - - - 100 1832.7 2 0 2 - - - - 0.07 -
3344.1 9 8 1 - - - - - - - 2059.2 3 0 2 0.08 0.14 - - - -
2204.8 4 0 2 0.06 - - - - -
a) Calculated mass of [M+H2PO4]- ion. Experimental masses were within ± 0.1 mass units.
b) Compounds are listed in order of increasing mass of the neutral glycans. The masses for acidic glycans found in each sample are listed after the neutral structures. Compounds not found as neutral structures are not listed.
c) H = hexose, N = GlcNAc, F = fucose.
d) Source of FSH preparations: P = pituitary, U = urinary, G = recombinant GH3 cell medium, F24 = pituitary hFSH24, F21 = pituitary hFSH21, and F21/18 = hLH-associated hFSH21/18.
e) The numbers represent the % of total identified glycans. T = trace.
e) Structures of the neutral glycans as shown in Figure 3.
f) Numbers in bold represent glycans whose structures have been confirmed by CID.
g) Si - Neu5Ac (sialic acid), SO3H = sulfate.
h) 1 = [M-H]-, 2 = [M-H2]2-.
Table 1: Masses and abundances of N-glycans found in pituitary, urinary and recombinant hFSH preparations compared with pituitary hFSH glycoform preparations.