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Figure 2: Migration assay. A: (Scratch assay) a: Control (untreated), b: AGE (100 μg/ml) treated c-e : Glycine (0.5,1 and 2.5 mM)+AGE, f-h: Glutamic acid (0.5,1 and 2.5 mM)+AGE (100 μg/ml),There is inhibition of migration by both the amino acids. B: Transwell migration assay: B (i) A: control (untreated), B: AGE (100 μg/ml) treated C: Glycine (2.5 mM)+AGE, d: Glutamic acid (2.5 mM)+AGE. B (ii) Bar graph of the cells counted in transwell assay is represented here and there was a significant inhibition of migration by glycine. |