No. Uniprot Accession Name Identified Glycopeptides HepG2 HZ HepG2 HA L02 HZ L02 HA 1 O00754 Lysosomal alpha-mannosidase AnLTWSVK     2   2 O00754 Lysosomal alpha-mannosidase LnQTEPVAGNYYPVNTR   6 7   3 O75882 Attractin IDSTGnVTNELR   1 4   4 P07996 Thrombospondin-1 VVnSTTGPGEHLR 4 2 4 4 5 P11717 Cation-independent mannose-6-phosphate receptor MnFTGGDTcHK   2 2 1 6 P11717 Cation-independent mannose-6-phosphate receptor mSVINFEcnK-TA     6   7 P11717 Cation-independent mannose-6-phosphate receptor nGSSIVDLSPLIHR   2   2 8 P11717 Cation-independent mannose-6-phosphate receptor TnITLVcKPGDLESAPVLR     2 1 9 P16070 CD44 antigen AFnSTLPTMAQMEK 2 9 6 8 10 P20645 Cation-dependent mannose-6-phosphate receptor EAGNHTSGAGLVQInK-SN 6 7 7 6 11 P20645 Cation-dependent mannose-6-phosphate receptor LnETHIFnGSNWIMLIYK       6 12 P32004 Neural cell adhesion molecule L1 DLQAnDTGR 2   3   13 P32004 Neural cell adhesion molecule L1 FFPYAnGTLGIR     2   14 P32004 Neural cell adhesion molecule L1 GYnVTYWR 1   2 2 15 P32004 Neural cell adhesion molecule L1 THnLTDLSPHLR   2 2 2 16 P32004 Neural cell adhesion molecule L1 VPGnQTSTTLK 1 3 6 4 17 P35613 Basigin ALMnGSESR 2 2 1 2 18 P35613 Basigin ILLTcSLnDSATEVTGHR   6 4 5 19 P35613 Basigin ITDSEDKALmnGSESR 5 6 8 4 20 Q12841 Follistatin-related protein 1 GSnYSEILDK 2   2   21 Q15155 Nodal modulator 1 LEnITTGTYTIHAQK 3   5   22 Q15155 Nodal modulator 1 ENVGIYnLSK 2 1     23 Q9UBG0 C-type mannose receptor 2 WNDSPcnQSLPSIcK   4   4 24 Q9UBG0 C-type mannose receptor 2 KKPnATAEPTPPDR 2 2     25 Q9UBG0 C-type mannose receptor 2 KPnATAEPTPPDR 2       26 Q9UBG0 C-type mannose receptor 2 TSnISKPGTLER 5 4 4 4 27 Q9UBG0 C-type mannose receptor 2 VTPAcnTSLPAQR 2 2 3 2

“n” represents the identified N-linked glycosylation sites; “-” represents the tryptic cleavage site. b) Numbers of corresponding spectra for each peptide with each method.HZ- hydrazide chemistry; HA- hydrophilic affinity

Table 1: Identified N-linked glycoproteins with carbohydrate binding ability.