The subcutaneous adipose tissue gene expression of CD86 and CD163 was assessed by quantitative real-time PCR in 57 non-diabetic individuals classified based on body mass index (BMI) as lean, overweight and obese as described in Methods. CD86 mRNA expression was found to be significantly higher in obese as compared with lean subjects (P = 0.0035) (A); however, the positive correlation with BMI (r = 0.25) did not reach statistical significance (P = 0.08) (B). Similarly, CD163 mRNA expression was also found to be higher in obese as compared with lean subjects (P = 0.0028) (C), which correlated positively with BMI (r = 0.255 P = 0.049) (D).
