Figure 2 Higher CD86 protein expression in the adipose tissues from obese individuals with or without type-2 diabetes. CD86 protein expression in the adipose tissues of non-diabetic and diabetic individuals was determined by immunohistochemistry (IHC) and/or confocal microscopy. The representative IHC photomicrographs show CD86 protein expression (arrows) in lean, overweight, and obese individuals, 2 each from 5 independent stainings in non-diabetics (A) and diabetics (B). The elevated CD86 protein expression was also confirmed by confocal microscopy in randomly selected lean, overweight, and obese samples from non-diabetic individuals; shown 1 each from 3 independent stainings (C).