Connectivity type

In human milk PLG?

Enzyme required

Host: secretory or external membrane?

Enzyme produced by GIT microbiota?

NeuAc-α-2,3-Gal

✓[42, 56, 84]

α-2,3-neuraminidase

✓ [18, 88, 89]

NeuAc-α-2,6-Gal

✓[40, 41, 43, 58]

α-2,6-neuraminidase

✓ [89]

NeuAc-α-2,6-GlcNAc

α-2,6-sialidase

✓ [89]

Fuc-α-1,2-Gal

✓[56, 57, 85]

α-1,2-fucosidase

✓ [18, 88]

Fuc-α-1,3-Glc

α-1,3-fucosidase

✓[83, 88]

Fuc-α-1,3-GlcNAc

 ✓[40, 41, 43, 56, 57, 84, 85]

α-1,3-fucosidase

✓[83, 88]

Fuc-α-1,4-GlcNAc

 ✓[56,57, 60, 85]

α-1,4-fucosidase

✓[83, 88]

Gal-β-1,3-Gal

✓[43]

β-1,3-galactosidase

✓ [18]

Gal-β-1,3-GalNAc

✓[42, 56, 60, 84]

β-1,3-galactosidase

✓ [18]

Gal-β-1,3-GlcNAc

✓[57, 57, 60, 85]

β-1,3-galactosidase

✓ [18]

GlcNAc-β-1,3-Gal

✓[41, 56, 60]

β-1,3-N-acetylglucaminidase

✓ [18]

Gal-β-1,4-Glc

β-1,4-galactosidase

✓ [18]

Gal-β-1,4-GlcNAc

✓[40-43, 56-60, 84, 85]

β-1,4-galactosidase

✓ [18]

Fuc-α-1,6-Gal

✓[43]

α-1,6-fucosidase

Fuc-α-1,6-GlcNAc

✓[40, 43, 58, 59]

α-1,6-fucosidase

GlcNAc-β-1,2-Man

✓[40, 43, 58, 59]

β-1,2-N-acetylglucaminidase

GlcNAc-β-1,4-GlcNAc

✓[40, 43, 58, 59]

β-1,4-N-acetylglucaminidase

GlcNAc-β-1,4-Man

✓[59]

β-1,4-N-acetylglucaminidase

GlcNAc-β-1,6-Gal

✓[56, 60]

β-1,6-N-acetylglucaminidase

GlcNAc-β-1,6-GalNAc

✓[42, 56] [60, 84]

β-1,6-N-acetylglucaminidase

Man-α-1,3-Man

✓[40, 43, 58, 59]

α-1,3-mannosidase

Man-α-1,6-Man

✓[40, 43, 58, 59]

α-1,6-mannosidase

Man-β-1,4-GlcNAc

✓[40, 43, 58, 59]

β-1,4-mannosidase

Table 1: Glycan bonds in human milk protein-linked glycans, enzymes that cleave these bonds, and whether those enzymes are secreted by bacteria within the human gastrointestinal tract. X in Connectivity type means the monosaccharide linkage was unspecified in literature.