Abbreviations: GlcCer: Glucosylceramide; LacCer: Lactosylceramide; Gb3: Globotriaosylceramide; SM: Sphingomyelin B. The data represent the mean ± SEM from 3 experiments. *denotes p< 0.05 calculated by two-way ANOVA of GraphPad Prism (n = 3).
Figure 1: Depletion of GSLs from cultured ECV304 cells with sphingolipids synthesis inhibitors: Cellswere treated in serum-free medium for 48 h. GSLs were extracted, purified and analyzed by high performance thin layer chromatography as described in the Methods section. A. A representative thin layer chromatography (TLC) plate of ECV304 cells treated with Et-DOP4, FB1 and myriocin at the indicated concentrations.