Figure 3: The depletion of GSLs induces deoligomerization of caveolin-1 in cultured ECV304 cells: Total cellular proteins were lysed with 1% Triton X-100, quantified using BCA assay, separated electrophonically in a 6- 12% gradient SDS-PAGE, and analyzed by immunoblotting in nonreducing and non-heating conditions. A. The Western blot is representative of three independent experiments that yielded equivalent results. B. the densitometric data summarized from the immunoblotsof the individual experiments.