Figure 4: GSL depletion eliminates high molecular weight oligomers of caveolin-1 in caveolar fractions of cultured Hela cells: Equal amounts of the initial Opti-prep gradient fractions were loaded into a 6-12% gradient SDS-PAGE. Caveolar caveolin-1 was verified by immunoblots with a polyclonal antibody against human caveolin-1 and compared to immunoblots using monoclonal antibody against human CD151 as a control.