Figure 1: (-) Nano-HPLC/MS analysis of human serum gangliosides.
(A) Display of extracted ion chromatograms (EIC) calculated for the most intense signal in the ceramide pattern of individual ganglioside glycoforms. Insert zooms into a set of less abundant analyte ions (upper line), a detailed list of detected ions is given in Table 2.
(B) Mass spectrum of GM3 ions eluting at min 35. (C) Mass spectrum of GD1a ions corresponding to an elution time of 44 min. doubly charged ions are marked by#, asterisks indicate partially coeluting VI3Neu5Ac-nLc4Cer species.