Normal liver Hepa 1-6 liver tumor Hepa 1-6 subskin tumor Hepa 1-6 HepG2 BNL Sham operation Regenerating liver
Structure Mean (%) SD Mean (%) SD Mean (%) SD Mean (%) Mean (%) Mean (%) Mean (%) SD Mean (%) SD
High-mannose type                          
GM9 2.3 0.1 1.1 0.2 1.3 0.2 1.1 2.2 2.1 1.0 0.4 1.1 0.0
M9A 14.0 0.3 8.9 0.8 7.6 0.6 7.8 8.1 7.7 14.9 2.3 15.8 0.7
M8A 15.9 0.8 10.4 0.6 10.7 0.5 9.7 18.0 7.4 16.1 4.0 14.9 1.1
M7A 3.3 0.5 2.5 0.2 5.5 1.1 5.4 3.9 4.6 3.0 0.3 2.9 0.2
M7B 4.0 0.4 2.6 0.4 3.4 0.9 2.7 4.2 2.0 3.0 1.3 2.7 0.2
M6B 7.4 0.6 6.4 0.6 5.5 0.6 8.0 5.3 9.6 8.4 1.4 8.8 0.4
M5A 5.7 0.9 4.9 0.6 6.0 0.2 4.1 7.3 3.9 6.3 1.7 7.3 0.8
Hybrid type                          
Gal-H5 ND - ND - 3.6 0.5 5.4 2.9 1.6 ND - ND -
Complex type                          
A2G0 1.3 0.4 ND - ND - ND ND ND 1.4 0.5 1.7 0.3
A2G0F 1.5 0.4 ND - ND - ND ND ND 1.0 0.7 1.3 0.2
A2G2 13.4 0.2 24.1 1.8 8.5 0.5 7.7 5.2 6.9 15.2 1.4 19.0 1.3
A2G2F 4.3 1.2 8.9 0.3 8.2 0.5 10.1 7.2 6.4 4.4 2.0 4.8 0.5
A2G2FB ND - 0.6 0.0 1.9 0.2 1.1 ND ND ND - ND -
A'3G3 3.3 0.3 5.4 0.7 2.9 0.2 ND ND ND 4.1 1.0 5.4 0.8
A3G3 ND - 0.6 0.1 1.3 0.2 5.3 3.2 4.4 ND - ND -
A3G3F ND - 0.5 0.1 1.1 0.3 1.3 ND ND ND - ND -
A3G2G'1(4) ND - ND - ND - 2.5 1.3 3.3 ND - ND -
A4G4F ND - 0.4 0.1 1.1 0.2 2.1 2.0 ND ND - ND -
Others                          
M4A 0.9 0.2 ND - ND - ND ND ND 0.7 0.2 0.5 0.1
M4B 1.8 0.5 1.1 0.2 2.2 0.3 ND 1.9 ND 1.1 0.2 1.4 0.3
M3B 2.2 0.4 1.4 0.1 2.9 0.2 2.0 5.6 1.6 1.6 0.2 2.1 1.0
A0G0F 1.1 0.4 1.6 0.3 ND - 2.7 ND ND 1.9 1.4 0.9 0.2
GnT V products 3.3 0.3 5.8 0.8 4.0 0.4 2.1 2.0 ND 4.1 1.0 5.4 0.8
GnT IV products ND - 1.5 0.3 3.5 0.7 11.2 6.5 7.7 ND - ND -
Table 2: N-glycans expressed in mouse normal livers, hepatomas, regenerating livers, and in liver metastasized or subcutaneously transplanted Hepa 1-6 cells or HCC cell lines. Amounts of major N-linked sugar chains expressed in normal or regenerating livers of three 9W mice together with those from three sham operated mice were measured. The amounts of N-linked sugar chains exceeding 1% of the total amounts did not vary significantly among the samples, and the standard deviations (SD) of each sugar chain level were extremely small. Amounts of N-linked sugar chains expressed in liver metastasized or subcutaneously transplanted Hepa 1-6 cells were analyzed, together with Hepa 1-6, BNL and HepG2 hepatoma cell lines in culture. ND, not detected.