Figure 1: SDS-PAGE of purified pig kidney Na+/K+-ATPase and the timecourse of deglycosylation with PNGase F. Lane 1: molecular-weight marker, lane 2: Na+/K+-ATPase purified from pig kidney, lanes 3-6: Na+/K+-ATPase treated with PNGase F for 0.5 h (lane 3), 1 h (lane 4), 2 h (lane 5) and 6 h (lane 6).