Name |
Oligonucleotide sequence |
Annealing temperature |
Second primer |
Product size (bp) |
Amplification strategy |
A. Primers used in the initial multiplex PCR amplifications |
Ex1Fw |
AGG ACA AAG TCT CCT AAT CTT C |
|
|
750 |
|
Ex1Rv |
CAA TGA TCT CTT GTA ACA TTG TAC |
|
|
|
Ex2Fw |
TAA CTG TAT CTC CTT TTC TAG C |
|
|
533 |
|
Ex2Rv |
AGG TCA GTG ATA GAG AGT ATG |
|
|
|
Ex4 Fw |
AAG TGT TTT ATA TCT AAT GTT TAC |
|
|
304 |
|
Ex4 Rv |
CTT CAG GGC TTG GTC AAT ATA G |
|
|
|
EX5Fw |
CAG AGC TTG GCA TAT TGT ATC |
|
|
321 |
|
Ex5 Rv |
GTA AAC ACA CAA CTA GTC AAT G |
|
|
|
Ex7 Fw |
TGT TCC ATT TCT CTC CTT TTC C |
|
|
272 |
|
Ex7 Rv |
GAA CAT GGA GTT GCA GTG TAG |
|
|
|
Ex8 Fw |
CAT ATT AAC TTC CCT CAC TTC |
|
|
174 |
|
Ex8 Rv |
CTT GTA CCC TGA AAC ACA AAG |
|
|
|
Ex9 Fw |
AGT AAC TTC TCC CTA TGT TTG |
|
|
312 |
|
Ex9 Rv |
GAT GAC GGG TCA GAA GAA G |
|
|
|
B. Allele specific Primers |
rs28399504, c.1A>G |
ACA AGA GGA GAA GGC TTC AA
ACA AGA GGA GAA GGC TTC AG |
61 |
Ex 1Rv |
288
134 |
Duplexed with § |
c.395G>A, rs72552267 |
CTC CCT CAT GAC GCT GCG
CTC CCT CAT GAC GCT GCA |
Ex 2Rv |
c.50T>C, rs55752064 |
CTC TCA TGT TTG CTT CTC CC
CTC TCA TGT TTG CTT CTC CT |
61 |
Ex 1Rv |
239
298 |
Duplexed with § |
c.1297C>T, rs56337013 |
ATG TTT GTT ATT TTC AGG AAA AC
ATG TTT GTT ATT TTC AGG AAA AT |
Ex9 Rv |
c.55A>C, rs17882687 |
CAG AGC TCT GTC TCC AGA T
CAG AGC TCT GTC TCC AGA G |
61 |
Ex1Fw
Ex8Rv |
554
122 |
Duplexed with § |
c.1228C>T, rs17879685 |
CCA GAG ATG TTT GAC CCT T
CCA GAG ATG TTT GAC CCT C |
c.99C>T, rs17885098 |
AAT CAC TGG GAG AGG AGT G
AAT CAC TGG GAG AGG AGT A |
61 |
Ex1Fw |
598 |
Duplexed with § |
c.636G>A, rs4986893 |
GTA AGC ACC CCC TGG
GTA AGC ACC CCC TGA |
Ex4Rv |
109 |
c.276G>C, rs17878459 |
GCC TCT TCC AGA AAA CTC G
GCC TCT TCC AGA AAA CTC C |
63 |
Ex2Fw |
147
488 |
Duplexed with ‡ |
c.449G>A, rs58973490 |
ACT CCT CCA CAA GGC AGC
ACT CCT CCA CAA GGC AGT |
c.431G>A, rs17884712 |
GAG GAG CAT TGA GGA CCG
GAG GAG CAT TGA GGA CCA |
61 |
Ex2Rv |
98 |
With amplicons from PCR A+B‡ |
c.681G>A, rs4244285 |
CTA TCA TTG ATT ATT TCC CA
CTA TCA TTG ATT ATT TCC CG |
57 |
Ex5Rv |
231 |
With amplicons from PCR A+B§ |
c.680C>T, rs6413438 |
CCA CTA TCA TTG ATT ATT TCC C
CCA CTA TCA TTG ATT ATT TCC T |
61 |
Ex5Rv
Ex7Fw |
234 |
Duplexed with § |
c.991A>G, rs3758581 |
GCT CCG GTT TCT GCC AAT
GCT CCG GTT TCT GCC AAC |
95 |
c.1251A>C, rs17886522 |
ACT TTC TGG ATG AAG GTG GC
ACT TTC TGG ATG AAG GTG GA |
61 |
Ex8Rv
Ex9Fw |
100 |
Duplexed with ‡ |
c.1473A>C, rs55640102 |
CAG ACC ATC TGT GCT TCT T
CAG ACC ATC TGT GCT TCT G |
231 |