Figure 3: Dose-dependent fold changes of selected miRNAs altered by treatment with APAP at 24h. The Welch’s t-test (p < 0.01) was used to identify the significantly changed (ΔCt value) urinary miRNAs at 24h after 1250 mg/ kg APAP dosing. Fold change was compared between APAP treated (100 mg/ kg or 1250 mg/kg) to the control group, calculated as fold change = 2[-(mean of ΔCt values of treated samples - mean of ΔCt values of control samples)]. The values are the average of at least 4 independent samples and the standard errors are shown as plus error bars.