Figure 6: miRNA biomarker is more sensitive and consistent than ALT for liver injury. miRNA-291a-5p fold change was compared between APAP treated (100 mg/kg or 1250 mg/kg) and the control group, calculated as fold change = 2[-(ΔCt value of treated sample - mean of ΔCt values of control samples)]. ALT fold change was compared to the mean ALT value in the control group.