Animal #

 

Activity, (IU/L)

 

Histopathology

 

 

ALT

AST

Centrilobular Glycogen Depletion

Centrilobular Necrosis

 

Control

1

69

114

0

0

2

65

90

0

0

3

71

105

0

0

4

65

83

0

0

MEAN +/- SD

67.5 ± 3.0

98.0 ± 14.1

0

0

 

 

 

 

 

 

 

100 mg/kg APAP

5

47

91

0

0

6

55

122

0

0

7

59

120

0

0

8

59

76

0

0

 MEAN +/- SD

55.0 ± 5.7

102.2 ± 22.5

0

0

 

 

 

 

 

 

 

1250 mg/kg APAP

9

59

127

1

0

10

1523

7580

4

3

11

69

131

1

0

12

71

108

2

1

13

65

117

2

2

14

68

103

1

0

15

1364

12880

4

3

 MEAN +/- SD

459.9 ± 673.5*

3006.6 ± 5166.3

2.1 ± 1.3

1.3 ± 1.4


Note: Rats were administered a single oral gavage dose of APAP and serum was collected 24 h after dosing. Histopathology lesions were scored on a 5 point scale (0 = normal, 1 = minimal, 2 = mild, 3 = moderate, 4 = marked).
*Statistically significant increase compared to the low dose APAP (100 mg/kg) group (p ≤ 0.05). Histopathology scores were not statistically analyzed.
Table 1: Inter-individual variability in traditional biochemical and histopathological indices of hepatocellular injury 24h after a single oral dose of APAP.