sequence
probe 1
5´-FAM-CTGCTGAGAACTGTAAGT-MGB-3´
probe 2
5´-VIC`-CTGCTGAGAACTTTAAGT-MGB-3´
forward primer
5'-TGTTGTGATGGGCACTCTGAC-3'
reverse primer
5`-TCATAGTTGTTGCAAGCCGAA-3`
Artificial templates
positive control (C allele)
5'-TCATAGTTGTTGCAAGCCGAACTGCT GAGAACTGTAAGTGTCAGAGTGCCCAT CACAACA-3'
positive control (A allele)
5'-TCATAGTTGTTGCAAGCCGAACTGC TGAGAACTTTAAGTGTCAGAGTGCCCA TCACAACA-3'