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Figure 5: Amalgam ion-features are formed when two monomers of abundant and closely eluting biochemicals adduct with one another forming a unique “dimer”. Panel A shows the XIC for a biochemical at m/z 205 in positive ESI and Panel B shows the XIC for a biochemical at 210 m/z in positive ESI. Panel C shows the XIC for the amalgam ion which is a dimer of one monomer of the 205 ion and one monomer of the 210 ion. Panel D shows the positive ion iontrap MS/MS spectrum of the 414 m/z amalgam ion where the fragment ions are the individual monomer masses. |