Figure 2: Unresponsiveness of RPM-EP cells to T-oligo treatment: A. FACS analysis was carried out using propidium iodide staining after treatment with 40 μM of either T572 oligo or c-oligo and diluent for 96 hrs. Percentage cell death was evaluated by determining the sub G0/G1 DNA content. No difference in cell death was observed between the treatment conditions. B. RPM-EP cells were treated with 40 μM of either T- oligo or c-oligo and diluent for 72 hours and TUNEL assay was performed. Since no shift in peaks was seen after treatment, there was no induction of apoptosis after T-oligo or c-oligo treatment.