Figure 3: RPM-EP cells were unresponsive to T-oligo treatment. RPM-EP cells were treated with 40 μM of either diluent, T-oligo or c-oligo for 48 and 72 hours. Western blot analysis was performed to evaluate the expression of E2F1, p73, Tyrosinase and TRP-1. A. No change in expression of E2F1 was observed and p73 was not detected. B. No change in expression of tyrosinase was observed and TRP-1 was not detected. ß-actin was used as the loading control.