Figure 1: Effect of AICAR, globular adiponectin (gAd), and creatine on glucose uptake by L6 myotubes. (A) Effect of AICAR on glucose uptake. L6 cells were incubated with low (0.5 mM) or high (5 mM) glucose. When present, AICAR was included at 1 mM and the uptake of glucose measured as described under “Materials and methods”. (B) Effect of gAd on glucose uptake in L6 cells. L6 cells were treated with or without 2.5 μg/ml of gAd for 2 h and then glucose uptake was measured. (C) Effect of creatine on glucose uptake in L6 cells. L6 cells were preincubated with or without 0.5 mM creatine for 2 days. After 2 h serum free, L6 cells were treated with or without 10 mM creatine in 0.5 or 5 mM glucose for 2 h, and then glucose uptake was measured. Each bar represents the mean ± S.E.M. * P< 0.05 v. 0.5 mM Glu and 5 mM Glu, respectively. Glu, glucose; gAd, globular adiponectin; Cr, creatine.