Figure 5: Effect of AICAR, gAd and creatine on AMPK phosphorylation by L6 myotubes (A) Effect of AICAR on AMPKα phosphorylation in L6 myotubes. L6 cells were treated with various concentrations of AICAR for 1 h, and phosphorylated AMPKα at Thr172 and AMPKα were measured by western blot assay. (B) Effect of gAd on AMPKα phosphorylation in L6 myotubes. L6 cells were treated with various concentrations of gAd for 2 h, and phosphorylated AMPKα at Thr172 and AMPKα were measured by western blot assay. (C) Effect of creatine on AMPKα phosphorylation in L6 myotubes. L6 cells were treated with various concentrations of creatine, and phosphorylated AMPKα at Thr172 and AMPKα were measured by western blot assay. AICAR was included for comparison. (D) Quantification of data of AICAR, gAd, and creatine on AMPKα phosphorylation. AMPKα was used as a control. Quantification studies were performed using the Image Analysis Tools of Fluor Chem™ (Alpha Innotech Corpor-ation, CA). * P < 0.05 v. control. P-AMPKα, phosphorylated AMPKα; gAd, globular adiponectin; Cr, creatine.